Anti-cross-contamination, txhim khu kev qha DNA kuaj tuaj yeem qhia qhov tseeb hauv qhov chaw zais

Kev tiv thaiv kab mob sib kis 86

Hauv ntau cov yeeb yaj kiab thiab kev sau ntawv, cov chaw soj ntsuam kev tshawb fawb pom tau tias muaj qhov tshwj xeeb thiab tseem ceeb, tshwj xeeb tshaj yog cov DNA txheeb xyuas cov phiaj xwm feem ntau dhau los ua tus yuam sij kom tau txais cov lus qhia thiab daws teeb meem.Txawm li cas los xij, yog tias qhov tseeb ntawm qhov kev ntsuam xyuas tau nthuav tawm yog qhov tsis ntseeg, nws yuav tsis dhau los ua pov thawj kev cai lij choj, cia nyob ib leeg qhia qhov tseeb hauv qhov chaw zais.Muaj ib qho tshwj xeeb ntawm lub cev thiab tshuaj lom neeg uas cov chaw soj ntsuam kev soj ntsuam yuav tsum tau kho nrog ceev faj, thiab qhov ntawd yog los tiv thaiv DNA cov qauv kuaj kom tsis txhob muaj kab mob hauv chav kuaj.Tam sim no, muaj ntau yam ua rau muaj kab mob DNA.Ntawm lawv, qhov muaj peev xwm ntawm kev sib kis kab mob yog qhov loj tshaj.
Kev tiv thaiv kab mob sib kis 781

Ib yam li lwm lub chaw soj nstuam, cov khoom siv thiab cov khoom siv hauv cov chaw soj ntsuam pov thawj tau kis kab mob ntau yam.Tshwj xeeb, kev sib kis ntawm DNA kuaj raws li PCR cov tshuaj tiv thaiv, lwm cov ntaub ntawv kuaj thiab cov pov thawj ntawm lub cev, thiab cov neeg sim lawv tus kheej yog qhov nyuaj tshaj plaws los kuaj xyuas.Cov kab mob sib kis no suav nrog cov kab mob lom neeg, ntshav, cov ntaub so ntswg, nrog rau kev sim tshuaj reagents, tshuaj ntxuav tais diav thiab lwm yam impurities.

Nws yog ib qho tsim nyog hais tias cov iav uas siv tau rov qab rau hauv cov chaw soj ntsuam xyuas xws li cov thawv ntim khoom, lub raj mis reagent, cov raj xa dej, cov raj xa dej, cov tais diav, petri tais diav, thiab lwm yam. culprits uas ua rau tsis ncaj ncees lawm kev txheeb xyuas thiab kev soj ntsuam cov lus xaus.

Anti-cross-contamination 1580

Hom iav iav no paug yog ib qho kev hem thawj rau cov txiaj ntsig kev xeem, yog li dab tsi yog qhov tseem ceeb los daws nws?

Ua ntej tshaj plaws, thaum pom tias muaj qhov teeb meem DNA hla kev sib kis, cov txiaj ntsig kev sim yuav tsum tau rov kuaj xyuas lub sijhawm kom rov qab tau qhov yuam kev.Qhov no yog qhov tseem ceeb thib ib.

Tom qab ntawd, ua kom muaj kev tswj xyuas qhov zoo ntawm cov khoom siv sim, suav nrog cov iav ntim, cov tshuaj reagents, thiab lwm yam, kom paub meej tias qhov chaw paug.

Rau lub hauv paus no, txhim kho cov txheej txheem ntxuav ntawm iav los kho qhov yuam kev, kom tsis txhob muaj qhov yuam kev zoo sib xws los ntawm kev tshwm sim dua.

Thib peb, tsuas yog los ntawm kev ntxiv dag zog rau tag nrho cov kev tiv thaiv kev ua qias tuaj thiab kev tsis sib haum xeeb ntawm lub chaw soj nstuam los tsim ib lub koom haum kev tswj hwm tuaj yeem ua kom zoo dua ntawm cov txheej txheem ntxuav kom zoo thiab muaj txiaj ntsig.

Qhov tseeb, ib lub chaw soj ntsuam kev soj ntsuam uas tsim nyog yuav tsum muaj thaj chaw mob siab rau thiab muaj kev ywj pheej rau kev kuaj DNA kom ntseeg tau tias cov pa phem raug txo qis hauv kev sib txuas sib txawv.Piv txwv li, cov ntaub ntawv lees paub thiab qhov chaw khaws cia, piv txwv DNA extraction cheeb tsam, DNA amplification cheeb tsam, DNA nrhiav kom tau cheeb tsam, qhov chaw soj ntsuam ua ntej, qhov tshwm sim tsom xam, cheeb tsam npaj, DNA amplification cheeb tsam, nrhiav tsis pom cheeb tsam, thiab hais txog.Ntawm lawv, kev ntxuav cov iav hauv qhov chaw npaj yuav pab tau kom muaj qhov tshwm sim tsis ua tiav ntawm qhov kev xeem.

Kev tiv thaiv kab mob sib kis 2954

Nws yuav tsum raug sau tseg tias ntau lub chaw soj nstuam tshaj lij, suav nrog cov chaw soj ntsuam kev tshawb fawb, tseem siv tsis tau cov txheej txheem kev ntxuav tes los daws cov teeb meem ntawm cov pa phem hauv iav.Tab sis txoj hauv kev no tsis yog tsim los txhim kho tib neeg txoj kev pheej hmoo ntawm kev sib kis.

Dab tsi ntxiv, qhov tsis zoo ntawm phau ntawv ntxuav ntawm iav mus deb dhau qhov no.

MKev tu txhua xyoo ntawm iav iav yuav tsis tsuas yog ua tsis tau kom huv si chav kuaj iav thiab cuam tshuam rau qhov kawg ntawm kev kuaj DNA thiab kev txheeb xyuas, nws tseem yuav ua rau muaj kev tsis sib haum xeeb xws li kev pov tseg ntawm cov peev txheej, kev ua haujlwm tu complexity, thiab kev nyab xeeb rau cov neeg ua haujlwm hauv chav kuaj. .Lub sijhawm no, kev siv ib qho automatic glassware washersiv nyob rau hauv thoob ntiaj teb forensic laboratories yuav pab txhim kho qhov teeb meem no.

Kev tiv thaiv kab mob sib kis 3773

Laboratory glassware washertuaj yeem ntxuav ntau yam khoom siv iav iav kom muaj kev nyab xeeb, batch, thiab ntse kom ua raws li cov kev cai cuam tshuam ntawm GMP thiab FDA.Piv nrog rau phau ntawv tu txoj kev, lub Laboratory Washertuaj yeem taug qab cov txheej txheem ntxuav thoob plaws tag nrho cov txheej txheem, uas yog qhov tsim nyog kom tau txais cov ntaub ntawv tseem ceeb hauv ntau yam.Cov ntaub ntawv no yog qhov tseem ceeb heev rau kev tshem tawm cov xwm txheej seem nrog rau kev sib kis los ntawm kev kuaj DNA.Tshwj xeeb tshaj yog thaum muaj qhov sib txawv thiab tsis ntseeg txog cov txiaj ntsig ntawm kev sim!

Nrog rau kev txhim kho txuas ntxiv ntawm cov thev naus laus zis uas cuam tshuam, cov chaw soj ntsuam kev ua pov thawj yuav ua lub luag haujlwm ntau dua hauv cov txheej txheem tuav ntaub ntawv.Nyob rau hauv txoj kev no, qhov yuav tsum tau ua rau txhua qhov kev sim ntsuas thiab cov qauv thiab qhov tseeb yuav nce ntxiv.Cov txheej txheem suav nrog kev kuaj DNA tsuas tuaj yeem lav cov txiaj ntsig huv thiab tau txais cov lus xaus thiab pov thawj tseeb yog tias lawv ua tiav hauv kev tshem tawm.Qhov no yog ib yam dab tsi uas txhua lub chaw soj nstuam yuav tsum nco ntsoov.


Post lub sij hawm: Apr-02-2021